The advice encourages marines to behave responsibly when sharing marine corps-related material online.
It adds that existing orders for the marines have "long prohibited" sexual or other harassment.
The Pentagon previously said sharing nude photos was "inconsistent" with its values.
"Marines should think twice before engaging in questionable online activities, and must avoid actions online that threaten the morale, operational readiness and security, or public standing of their units, or that compromise our core values," the new guidance says.
It also warns against posting discriminatory content that targets a person's "race, colour, sex, gender, age, religion, national origin, sexual orientation or other protected criteria".
Servicemen and women were advised to report any questionable content.
Earlier this month, top marine Gen Robert Neller told anyone participating in the activity: "You're not helping me or your marine corps."
Senator Kirsten Gillibrand, a New York Democrat, responded by saying there was still a need for the military to tackle longstanding allegations of rampant sexual assault and harassment.
A Facebook group where some of the nude photos were shared, Marines United, had 30,000 members but was shut down after the scandal hit headlines.
The BBC has seen a separate message board where servicemen from other branches of the US military have shared hundreds of nude photos.